Myriad of bacterial species that form gut micro flora is a complex ecosystem; altogether play a major role in the physiology of their host. Bifidobacteria are among the first species to colonise the human gastrointestinal tract (25% and 80% in adults and infants respectively) and as such declared they share a prominent role in gut homeostasis and normal development. Clinical properties of Bifidobacteria like prevention of diarrhea, atopic eczema, irritable bowel syndrome, pouchitis, alleviation of constipation, reduction of serum cholesterol and the status of GRAS ('Generally Recognized as Safe'). Bifidobacteria along with other probiotics offer a safe alternative or addition to current therapy for the above mentioned disorders. Interest in Bifidobacteria has burgeoned in last two decades in a fastidious way because of their potential use in food industry to relieve and in the medical field is increasingly attracting the scientific curiosity of clinicians and researchers. Bifidobacteria can be considered as valuable probiotics for their clinical properties. This review will enlighten on the Bifidobacteria and as potential probiotic agents for few disorders. © IDOSI Publications, 2012. © 2012 Elsevier B.V., All rights reserved.