PHF, a polyherbal formulation, consist of seven known herbs namely, Aegle marmelos, Elettaria cardamomum, Glycyrrhiza glabra, Citrus aurantifolia, Rosa damascena, Cissus quadrangularis and Saccharum officinarum. The PHF was evaluated for acute toxicity, gastrointestinal motility and gastric emptying rate in mice and rats. Based on acute toxicity study, the PHF was considered as safe and 3 dose (100, 200 and 400 mg/kg) levels were employed for further pharmacological studies. The gastrointestinal prokinetics effect of PHF in various dose levels (100, 200 and 400 mg/kg., p.o) was studied by charcoal meal gastrointestinal transit and laxative effect in mice. The gastric emptying rate of PHF in rat was studied by disappearance of phenol red from stomach. The results illustrate that PHF at 200 and 400 mg/kg significantly (p<0.001) enhanced the gastrointestinal transit. PHF at 400 mg/kg (p<0.01) significantly enhanced the purging index, which is the measure of laxative activity. PHF at 200 mg/kg (p<0.05) less significantly enhanced the purging index. In gastric emptying rate, PHF dose dependently increased the gastric emptying. From the above findings, PHF may be used as gastrointestinal prokinetics and to improve the intestinal motility. © Copyright 2009 Phcog.net. © 2010 Elsevier B.V., All rights reserved.