Aim: The aim of the study was to compare the level of awareness on Hepatitis-B infection among medical and dental interns in Pondicherry, India. Materials and Methods: Questionnaire containing a set of 20 different questions on Hepatitis B infection was distributed among 64 Medical and 49 Dental Interns of Arupadai Veedu Medical College and Mahatma Gandhi Dental College, Pondicherry, India, respectively. Results: The majority of interns (94.7%) were aware that Hepatitis B virus is the major cause of Hepatitis. About 33.6 and 59.3% of interns had very good and moderate knowledge respectively. The study shows that dental interns (34.7%) had better awareness than medical interns (32.8%). About 52.2% believed that Hepatitis B virus can be transmitted by semen and vaginal secretions. Only 39.9% of the interns believed that doctors, dentists infected with HBV should not have direct contact with patients. Conclusion: One-third of the interns had very good knowledge about Hepatitis B. The majority had good knowledge regarding the mode of transmission and attitude towards HBV patients. However, there is a misconception about prophylaxis, vaccination and treatment of HBV. Hence there is an imperative need for health education to improve the knowledge and attitude of the interns towards Hepatitis B. © 2010 Elsevier B.V., All rights reserved.