A rapid, sensitive and specific RP-HPLC method involving UV detection was developed [1-15] and validated [16-21] for determination and quantification of Amoxycillin and Potassium Clavulunate. Chromatography was carried out on a pre-packed Hypersil C18 (5μm, 250x4.6mm) column using filtered and degassed mixture of Phosphate buffer:Methanol (95:05) as mobile phase at a flow rate of 1.0ml/min and effluent was monitored at 220nm. The method was validated [16-21] in terms of linearity, precision, accuracy, and specificity, limit of quantification and limit of detection. The assay was linear over the concentration range of Amoxycillin and Potassium Clavulunate was 25mcg-200mcg/ml and 5mcg to 40mcg/ml respectively. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analyzed test solution and was found to be 97.70%-103.00% and 96.80%-102.01% within precision RSD of 0.432 and 0.988 for Amoxycillin and Potassium Clavulunate respectively. The system suitability parameters such as theoretical plates and tailing factor were found to be 3189.33, 1.225 and 7852.83, 1.08 respectively for Amoxycillin and Potassium Clavulunate. The method does require only 15 minutes as run time for analysis which prove the adoptability of the method for the routine quality control of the drug. © 2010 Elsevier B.V., All rights reserved.