The maximal mycelial growth recorded in glucose amended cuture, and in the Production and isolation of extra cellular carbohydrates and proteins the maximum amount of protein 231 mg/L was record in sucrose amended medium besides Ganoderma lucidium produced maximum amount of carbohydrate (975 mg/L) and protein (55.4 mg/L) in synthetic medium. The absorption peaks of polysaccharide isolated from the fruit body of G. lucidium coincide with the 1,3-β-D glucan (Sigma). Effect of polysaccharide on fibrosarcoma 180 induced mice. The body weight of sarcomainduced mice was increased by 11% than the normal mice. In polysaccharide treated mice the body weight significantly decreased and was similar to that of the normal mice, and remarkable increase in the weight of the spleen (336%) and kidney (10.6%) in sarcoma induced mice than normal mice whereas the weight of liver decreased to much as 29.3% in induced mice. In polysaccharide treated mice a remarkable percent of decrease in weigh of the liver (116%), spleen (53.1%) and kidney (35.46%) were observed. The concentration of DNA and RNA were elevated in induced mice. © 2010 Elsevier B.V., All rights reserved.