Samadera indica a bitter plant widely distributed throughout India. The bitterness is due to the presence of triterpenoid such as quassinoids, this group responsible for wide variety biological activities. In the current world population, incidence of infection is increasing tremendously and hence the present study was aimed to carry out the antimicrobial activity and antioxidant activity of methanolic extract of Samadera indica, both the activities can be used to predict the wound healing activity of this extract. The methanolic extracts of Samadera indica obtained by soxhletation and was investigated for in vitro antimicrobial activity against microorganism including Gram-positive (Staphylococcus aureus, Bacillus Subtilis), Gram-negative bacteria (Pseudomonas aerueginosa, Escherichia coli and Proteus Vulgaris) and few strains of fungus such as Candida albicans, Aspergillus niger and Aspergillus fumigates. In addition, evaluated antioxidant activity and estimated total phenolic and flavanoids content. Antimicrobial studies revealed that it has significant activity against gram positive, gram negative bacteria and Candida albicans, but was resistant against Aspergillus niger and Aspergillus fumigates. All the methods of antioxidant showed a prominent antioxidant activity and were compared with Quercetin. Antioxidant activity of extracts produced increased scavenging activity in a dose dependent manner. The present study revealed the presence of antimicrobial and antioxidant activity, hence further studies could be carried out to find out the wound healing activity. © 2012 Elsevier B.V., All rights reserved.