Self assembly provides a simple route to organise suitable organic molecules on noble metal surface by using long chain organic molecules with various functionalities like -SH,-COOH,-NH <inf>2</inf>, Silanes etc. These surfaces can be used to build up interesting nano level architectures. Self assembly is an interesting process for biological relevance because it provides a novel approach to complex structure having nanometre scale dimensions. The self assembled monolayers (SAMs) can be characterized by the number of techniques such as Contact angle goniometry, X-ray photoelectron spectroscopy, Infra red and scanning probe microscopy which provides the valuable information about the SAMs. © 2012 Elsevier B.V., All rights reserved.