Background & Objectives: P. aeruginosais a classical opportunistic pathogen with innate resistance to many antibiotics.The common form of resistance is mediated by lack of drug penetration and/or carbapenem- hydrolysing beta-lactamases. The metallobetalactamases (MBL) are enzymes requiring divalent cations as cofactors for enzyme activity, being inhibited by the action of a metal ion chelator. This study is designed to know the prevalence of MBL producing Pseudomonas in hospitalized patients. Methods: A total of 526 Pseudomonas isolates received in the microbiology laboratory over a period of six months were subjected to the study. Isolates with resistance or with intermediate sensitivity to imipenem(≤15mm) and/ or ceftazidime(≤ 17mm) were screened for MBL production. Results: Out of 526 Pseudomonas isolates, 116 isolates (22%) were positive for MBL production. Conclusion: Increased use of broad-spectrum antibiotics, intubation and intravascular catheterization are significant predisposing factors for development of antibiotic resistance. Various strategies such as strict infection control measures, judicious prescribing of antibiotics, antibiotic resistance surveillance programs and antibiotic cycling must be tried. Regular monitoring and documentation of carbapenem resistance is therefore crucial in developing strategies to control infections due to these bacteria. © 2013 Elsevier B.V., All rights reserved.