Ethanol Extract of Impatiens balsamina was investigated for anticancer and in-vitro cytotoxic activities against transplantable tumors and human cell line. In vitro cycotoxicity was evaluated in Hela and NIH3T3 cells by MTT assay and in-vivo antitumor activity with Dalton's ascites lymphoma (DLA) tumor bearing mice. Activity was measured by monitoring the Cancer Cell number, packed cell volume and increase in life span. Hematological parameters were evaluated and compared with the respective controls. The extract exhibited strong in vitro cytotoxicity against Hela cell line, it was found to be safe with normal cell. EEIB at the doses 200 and 400 mg/kg significantly increase the life span and decrease in the cancer cell number and body weight (P<0.01) and exert a protective effect on the hemopoietic system. The results showed significant antitumor and cytotoxic effects against DLA and human Cancer Cell line and support the ethno-medical use of Impatiens balsamina in cancer therapy. © 2014 Elsevier B.V., All rights reserved.