Rapidly emerging strains of bacteria resistant to most advanced antibiotics have become issues of very important public health concern. Research currently directed towards marine actinomycetes presents a vast potential for new compounds that could be able to safely and effectively target resistant species. In this regard seven putative Streptomyces strains were selected and assessed for antibiotic production and activity against a wide range of plant fungal pathogen (Rhizoctonia solani) and human pathogenic bacteria (Staphylococcus aureus, Vibrio cholera, Salmonella typhi, Klebsiella pneumonia). In conclusion, the secondary metabolites of marine organisms displayed appreciable antimicrobial activity. The secondary metabolites were of low molecular compound with stable free radical scavenging activity. The compound(s) present within this extract showed activity against Staphylococcus aureus, Vibrio cholera, Salmonella typhi, Klebsiella pneumonia. The antagonistic secondary metabolite produced by this actinomycetes needs to be studied further to identify its chemical nature and characterization of its biological activity. The chromatogram developed with 10% ethyl acetate in hexane revealed the presence of ten major compounds at R<inf>f</inf> value of 0.21, 0.33, 0.68, 0.78, 0.78, 0.88, 0.90, 0.95, 0.95 and 0.96 as visualized under iodine vapour and UV illumination. However, further studies are undertaken to purify, characterize and test the active molecule for its antibacterial activity and its mechanisms of action. © 2013 Elsevier B.V., All rights reserved.