Tumor Necrosis Factor-alpa (TNF-A) is a cytokine critical for effective immune surveillance wherein cytokines are usually associated with inflammatory process which in turn causes many clinical problems associated with autoimmune disorders. The present investigation was aimed to generate a combinatorial library with eight TNF inhibitors in terms of creation, physico chemical characterization, alignment, 2D QSAR analysis and bar graph visual pattern for chemical diversity among the molecule using Vlife MDS3.5. © 2013 Elsevier B.V., All rights reserved.