Objective: This prospective observational study was conducted to assess the prescribing pattern of antimicrobial agents in the MICU of a tertiary care teaching hospital in Puducherry Union Territory and to suggest necessary modifications in prescribing drugs to achieve rational therapeutic practices. Methods: 100 consecutive inpatient records of patients admitted to the MICU during September to December, 2013 were studied. Results: Average number of AMAs per patient was found to be 1.13. Cephalosporins and Ceftriaxone were the most frequently prescribed Anti microbial group and agent respectively in the MICU. Organisms isolated in the culture and sensitivity tests were sensitive to Imipenem and Piperacillin + Tazobactam in common. No adverse drug reaction was observed for any AMA. Brand name of the drugs and parenteral route of administration were preferred by the physicians for prescribing the AMAs. Most AMA prescriptions were made without bacteriological culture and sensitivity testing evidences. Conclusion: There is a need for motivating the physicians to prescribe antimicrobial agents by generic names with supportive bacteriological evidences. Antibiogram need to be framed and followed. © 2014 Elsevier B.V., All rights reserved.