The purpose of this research is to formulate and evaluate in-vitro evaluation of Bucoadhesive tablets of Midazolam microspheres for the buccal delivery of the drug. Drug was presented in the form of microspheres to impart site specific drug delivery. Delivery of drug to the buccal cavity avoids first pass metabolism and comparatively reaches brain quickly. Microspheres can increase the site specific delivery system and possible to cross the body fluids like BBB, CSF, etc. Tablets were prepared by incorporating drug loaded microspheres and using bioadhesive polymers HPMC K15M and Carbopol 934. Micropheres were prepared by the Emulsion Cross linking method using Eudragit E100 and Eudragit RL100. Microsphres were characterized by SEM, FTIR, and particle size analysis and evaluated for percentage yield, drug loading, encapsulation efficiency and in vitro drug release. To achieve bioadhesion to the mucosal tissue, optimized microspheres were incorporated into bioadhesive tablets and were evaluated for in vitro drug release, in-vitro and in-vivo mucoadhesion. FTIR study showed that no chemical interaction occurred between the drug and polymers. Formulation F11 indicated a controlled in vitro drug release and good bioadhesive strength. The results indicated that this drug delivery system can be explored for controlled buccoadhesive drug release. © 2017 Elsevier B.V., All rights reserved.