Background: Surface landmarks of the temporal bone are related to various anatomical structures. The surgical exposure of the middle cranial fossa, transpetrosal and transmastoid approaches for skull base surgeries would require identification of various surface landmarks for approaching the structures. Aim and Objectives: To determine whether Henle's spine could be used as a reliable and multipurpose landmark for the important structures and foramen of the skull base during surgeries. Materials and Methods: This study was done on 170 dry human skulls. Two imaginary lines and a parapetrosal triangle was constructed which encompasses nearly all the main structures and foramina of the skull base. The distance from the Henle's spine to certain useful surgical landmarks on the skull base was measured and tabulated. Results: The measurements were analysed and compared by student't' test both the sides and found to be have slight variations in the measurements of right and left. Distance from Henle's spine to stylomastoid foramen and jugular foramen were found to high when compared to earlier studies other measurements showed only a slight deviations. Conclusion: Henle's spine when present (85%) can be used as a reliable anatomical landmark for approaching various foramina during skull base surgeries when other land mark fails. © 2018 Elsevier B.V., All rights reserved.