Background: Facial anthropometrics are affected by various factors such as age, sex, ethnicity, socioeconomic status, environment and region. The anthropometric analysis of face is essential for craniofacial plastic and reconstructive surgeries. Aim & Objectives: The purpose of this study was to create a baseline data in determining the sex of the people from India and Malaysia depending on morphometry of eyes. Material and Methods: A total of 100 Malaysians and 100 South Indians were enrolled for the study. Various morphometric measurements of eyes were taken using Digital Vernier caliper. The data were analyzed by independent t test for comparison of sides and one way ANOVAto find out the significance among the sex and populations. Results:The height of upper and lower eye lid were higher in females as compared to that of males whereas depth of eyes and interpupillary distance were higher in males than in females and thus sexual dimorphism exists. Fissure height and biocular width were more in Indian males when compared to Indian females. Fissure height and interocular width were more in Malaysian female when compared to other groups. Indian males and females differed significantly in upper and lower eye lids height from Malaysian males and females. All eye morphometric parameters except interpupillary distance were increased in Malaysians when compared to Indians thus ethnic variations also exist. Conclusion: The facial aesthesis is highly contributed by eye appearance and symmetry, facial rejuvenation by a plastic surgeon requires morphometric data of eye. © 2019 Elsevier B.V., All rights reserved.