Background: Lingual gingival recession is a unique clinical concern because of its aesthetic and oral health implications. While many treatment approaches to root coverage have been explored, use of amniotic membrane (AM), known for its anti-inflammatory and regenerative capabilities, is comparatively less used in lingual recession defect treatment. Objective: The case series was done to assess the efficacy of amniotic membrane to achieve root coverage of lingual recession defects, with an image analysis system for objective measurement. Methods: Three patients with Cairo's RT I or II classification of lingual recession were included. After local anesthesia and meticulous root surface debridement, AM grafts were placed onto the recession areas and splinted with sutures. Postoperative assessments were performed over a period of six months. Clinical outcomes data were monitored using data such as recession depth (RD), recession width (RW), keratinized tissue width (KTW), mucosal thickness (MT), percentage of root coverage, and measurements that were further validated with an image analysis system for increased accuracy. Results: All cases showed significant root coverage and KTW gain at the six-month follow-up. Image analysis revealed a mean root coverage of 92%, with significant soft tissue healing and esthetic improvement. Patients reported minimal postoperative pain and were highly satisfied with the result. Conclusion: Treatment of lingual gingival recession using amniotic membrane produced satisfactory clinical and esthetic results, indicating its possibility as a good regenerative therapeutic modality in periodontal therapy. © 2025 Elsevier B.V., All rights reserved.