Green synthesis of metallic nanoparticles using medicinal plant extract treated to environmentally friendly, stable, cost-effective. Present work to study In-vitro antioxidant and cytotoxicity of green synthesised CuNPs using P. Pellucida plant extract. Copper nanoparticles were synthesised biogenically using P. Pellucida plant aqueous extract. CuNPs Characterised by using FE-SEM, U.V., FT-IR, EDAX. Biogenically synthesised copper nanoparticles conducted in-vitro Antioxidant (DPPH) and cytotoxicity (SK-MEL-3 cell). The results CuNPs were characterised by using U.V. spectroscopy absorbance 575 nm. Scanning electronic microscope showing the distribution and shape (5-20 nm) of the nanoparticles. X-ray spectrum showed different peaks for CuNPs detected at 2θ values 35.45°, 44.32°, and 65.25°. EDAX elemental spectroscopy conformed to the copper metal inside the nanoparticle (69.7%). The synthesised CuNPs showed good free radical scavenger activity than the ascorbic acid, and it also showed significant cytotoxicity (16μg/mL) against human skin cell lines (SK-MEL-3 cell). The current study conclusion recommended that green synthesised (P. Pellucida) CuNPs be used for therapeutic application. © 2023 Elsevier B.V., All rights reserved.