Introduction: It is well known that the biomineralization process leading to the synthesis of an interfacial layer with tag-like structures is due to the synergeticeffect of mineral trioxide aggregate (MTA) & Portland Cement with dentin in phosphate-buffered saline (PBS) at the cement-dentin interface. However, there are no investigations which have examined the effect of the influence of biomineralization process on the push-out bond strength of ProRoot MTA (Dentsply Tulsa Dental, Tulsa,OK), Biodentine & Calcium phosphate cement (BioGraft CPC). Aim: To examine the effect of biomineralization process on the push-out strength of ProRoot MTA, Biodentine & CPC after mixing with0.2% Chlorhexidine gluconate solution and 2% local anaesthetic solution on the bond strength of MTA-dentin. Materials and Methods: Dentin discs with uniform cavities were filled with ProRoot MTA, Biodentine & Calcium Phosphate Cement. The samples were equally divided into two groups: experimental group being immersed in PBS and control group being immersed in Saline for 2 months. Instron Testing machine (Model 4444; Instron Corp, Canton, MA) was utilised for determining the bond strength. Statistical analysis used: A two-way analysis of variance & post hoc analysis by Bonferroni test. Results: Specimens inserted into PBS showed a significant resistance to dislodgement than that detected for the samples of Saline (p < 0.05). MTA & Biodentine exhibited higher resistance to dislodgement than Calcium Phosphate Cements. Conclusion: It was concluded that the push-out bond strength of the cement mainly the MTA & Biodentine groups, were positively benefitted by the biomineralization process. © 2021 Elsevier B.V., All rights reserved.