Ayurveda, the Terminalia chebula (TC Terminalia Chebula Fruit Extract) herb, and especially its fruits dried pulp, are well-known. It is one of the important ingredients used in triphala or “three fruits”—the most frequently prescribed herbal drug remedy in ayurveda. Among Indian, The biochemical activities previously reported for water-soluble TC fruit extracts appear to be due to the presence of hydrolysable tannins; more specifically Ellagic acid, chebulinic and chebulagic acids. Ellagic acid has cytoprotective and cancer protective effect against various carcinogenic induced cancers cell. Some study on laboratory animals have pointed that periodical oral administration of ellagic acid in rats can circumvent the carbon tetrachloride (CCl<inf>4</inf>) toxicity and formation of fibrosis in the liver, METHOD Ellagic acid compound was purchased from Sigma-Aldrich Co. LLC, USA, Product Number: E2250. All other reagents were of HPLC grade and purchased from SDFCL. RESULTS: In this analytical study, a simple, precise, accurate and rapid reverse phase HPLC method has been established and validated for the determination of ellagic acid in herbal formulation. The percentage recoveries for the ellagic acid was 98.96%, 100.1%, 100.14%.DISCUSSION: The High-performance liquid chromatography method was validated to estimate the precision, repeatability and accuracy. The relationship of the mean concentration of standard solution and the average peak response was slightly linear with the solution concentration range variables of 20μg/ml to 120μg/ml with a desirable correlation coefficient of 0.999. The y= 43278x precision was found by continued repeated delivery of injections of the same concentration five times (% CV = 0.0572). The Accuracy level was determined at three levels (80%, 100% and 120%). The specific recoveries was found 98.96%, 100.1% and 100.14%. The phase detection of ellagic acid was calculated from its standard deviation of the linear curve and sl ope found 3.744μg/mL. © 2021 Elsevier B.V., All rights reserved.