The concrete structures normally have some capacity for self-healing, which means the concrete can able to seal or heal freshly formed micro-cracks which property mainly because of an excess of non-hydrated cement particles present in the materials. This reaction with ingress water undergoes secondary reaction. This paper carried out a new kind of self-healing concrete by using bacteria as mediate production of minerals which can seal the freshly formed cracks rapidly. The characteristics of self-healing bacterial concrete with M-sand and various dosages of Bacillus substilis, like 0 mL, 10 mL, 20 mL and 30 mL for the M25 grade concrete, in its fresh state are investigated by workability, while hardened characteristics are investigated by compressive, tensile strength, durability studies of acid test, like hydrochloric attack, sulphate attack and chloride attack and self-healing assessment. The study was carried out on fresh and hardened characteristics of a bacteria based self-healing concrete with M-sand using Bacillus subtilis bacteria. The test results indicated that by the addition of 20 mL amount of bacterial content in self-healing concrete with M-sand, the characteristics of durability performance was greatly altered and thus facilitate a sustainable environment. © 2022 Elsevier B.V., All rights reserved.