Abutilon hirtum (Lamp) Sweet is one of the species of abutilon, commonly known as Vadathuthi. Abutilon hirtum has antioxidant, and hepatoprotective properties. The present work involves the biosynthesis, characterization and in-vitro cytotoxic evaluation of silver nanoparticles of stems of Abutilon hirtum. Silver nanoparticles was synthesised by green method and were confirmed by various spectral studies. The UV/Vis spectra absorption peak confirms their production. scanning electron microscopy, transmission electron microscopy and energy diffraction X-ray analysis revealed that the silver nanoparticles are polygonal and of different morphologies ranging from 16 to 56 nm in size. The green synthesized AgNPs were evaluated for its in-vitro cytotoxicity against HepG2 cell lines The synthesized AgNPs showed significant cytotoxicity against HepG2 cell lines in a dose dependent manner. © 2022 Elsevier B.V., All rights reserved.