Introduction: the effect of Chlorophytum borivilianum extract against high fat diet induced obesity is unknown. Therefore, this study investigates activity of 250 & 500 mg/kg/day of Chlorophytum borivilianum extract in male Wistar Albino rats, on parameters like weight gain, Follicle Stimulating Hormone, Luteinizing hormone, and Testosterone. The objective is to determine the effects of Chlorophytum Borivilianum extract against high fat diet-induced obesity in male Wistar Albino rats. Materials and Methods: it’s an interventional, comparative, animal study of 60 days, conducted at KMCH college of pharmacy, Coimbatore. 20 male Wistar Albino rats were randomly divided into 5 experimental groups with 6 rats per group. At the end of experimental period, all rats were fasted overnight, weighted, and sacrificed within 24 hours by the mode of decapitation, and the blood was collected. Later, the organs were dissected out and fixed with formalin. The post-fixed tissue, slides were stained. Ethics committee approval was obtained initially. Statistical analysis was done by SPSS software. Results: the Obese group showed an increase in body weight due to high-fat diet administration, whereas, the treatment group receiving Chlorophytum borivilianum extract showed reduction in body fat and improvement in the semen analysis when compared to control group. Conclusion: when treated with Chlorophytum borivilianum, body weight as well as sperm function of the animals was significantly improved mediated through enhanced spermatogenesis, steroid genesis, and antioxidant mechanisms with histological studies. Thus, findings reinforce the advice recommending consumption of Chlorophytum borivilianum extract to modulate obesity and sperm morphology. © 2024 Elsevier B.V., All rights reserved.