Protocols for in vitro plant multiplication from somatic tissues and production of artificial seeds via node encapsulation were developed for Aristolochia tagala Cham., a rare medicinal plant, for conservation and genetic transformation. Maximum adventitious shoots regenerated from leaf-derived callus on MS medium with BAP (2 μM), NAA (0.5 μM), and phloroglucinol (PG; 10 μM). Nodes from in vitro shoots were encapsulated in 3% sodium alginate and 1% calcium chloride. Multiple shoots regenerated from encapsulated nodes on MS medium with 3 μM BAP and 0.5 μM kinetin. Shoots from callus and artificial seeds rooted successfully and acclimated to greenhouse. Direct root regeneration from leaf-derived callus was developed. Maximum root length on MS medium with KIN (1 μM), IAA (0.5 μM), NAA (0.1 μM), and PG (10 μM). Calli grown with PG showed increased antioxidant enzymes and metabolites, decreased polyphenol oxidase. © 2013 Springer Science+Business Media Dordrecht. © 2021 Elsevier B.V., All rights reserved.