Introduction: Human immunodeficiency virus is one of the greatest challenges faced by mankind. The adolescent cohort is one of the most vulnerable groups as far as risk of HIV/AIDS is concerned. Logically, teachers are the ideal sources of imparting information to the students. Aims and Objectives: 1) To estimate the knowledge regarding HIV/AIDS among high school teachers. 2) To compare the knowledge regarding HIV/AIDS of the science teachers with the knowledge of the non-science teachers. Material and Method: The study was done using a pretested structured questionnaire. The number of teachers participated in the study were 168 from 20 schools that were selected. The study was done in 3 months. Statistical Analysis: Percentages and Chi-Square test. Result: There is no statistical difference in the knowledge parameters between science and non-science teachers. © 2021 Elsevier B.V., All rights reserved.