Introduction: Pre-eclampsia (PE) is a major cause of maternal and fetal/neonatal mortality and morbidity. The aetiology and pathogenesis of PE is yet to be completely understood. Evidence shows that Endothelial Dysfunction (ED) plays a pivotal role in the genesis of this multi-system disorder that develops in PE and eclampsia. Aim: To determine the circulating levels of factors Malondialdehyde (MDA), Ferric Reducing Ability of Plasm-α (FRAP), Tumour Necrosis Factor (TNF-α), sFlt-1, VEGF, PlGF, Nitric Oxide (NO) that influence the ED. Materials and Methods: Study groups consisted of Normotensive pregnant women (N), preclamptic women (PE), and eclamptic women (E) with 100 subjects in each group in the 3rd trimester of pregnancy. They were investigated for MDA, FRAP, TNF-α, sFlt-1, VEGF, PlGF, NO. Statistical analysis was done using Analysis of Variance (ANOVA). Results: When compared to controls MDA, TNF-α, sFlt-1 levels were found to be significantly high and FRAP, VEGF, PlGF, and NO levels were significantly low in PE and E groups. E showed a significantly high level of MDA, TNF-α, sFlt-1 and low levels of FRAP, VEGF, PlGF, NO when compared to PE group. Conclusion: Our study substantiated that oxidative stress and imbalance between anti-angiogenic factors and proangiogenic factors exists in Pregnancy Induced Hypertension (PIH) condition. This imbalance is directly related to ED, the hallmark of PE. So oxidative stress, VEGF, PlGF, and sFlt-1 can be used as markers to analyze the onset and progression of the disease. © 2018 Elsevier B.V., All rights reserved.