The green synthesis of nanoparticles plays a vital role in biomedical applications due to its simplicity and environmental friendliness, as it involves the fabrication of nanoparticles from plants. In this study, the green synthesis of silver nanoparticles (AgNPs) was performed using the fruit extract of Alangium salvifolium (L.) as a capping and stabilizing agent. The green-synthesized AgNPs were characterized using various spectroscopic techniques. The UV–visible spectrum analysis revealed an absorbance peak at 430 nm, confirming the presence of the synthesized AgNPs. The FTIR spectrum showed the presence of various important functional groups in the nanoparticles. XRD results exhibited the high crystalline characteristic of AgNPs. FESEM analysis illustrated the spherical-shaped nanoparticles with an average particle size of 35 nm. The EDX spectrum and mapping study found that the nanoparticles contain silver (68.46%) and oxygen (20.18%). The antioxidant activity of AgNPs showed a strong ability to neutralize free radicals, with a result of 76.42 ± 0.26% for DPPH at a concentration of 200 µg/mL. AgNPs exhibited excellent antibacterial properties against Staphylococcus aureus (17.95 ± 0.73 mm) at a concentration of 200 µg/mL. The anticholinesterase activity analysis results showed that AgNPs had a substantial effect in blocking BChE (83.42 ± 1.29%) at a concentration of 200 µg/mL. A cytotoxicity effect was observed at a concentration of 200 µg/mL, with a value of 31.57 ± 0.72% against A549 cells. These study results confirm that Alangium salvifolium fruit extract-mediated green-synthesized AgNPs have various intriguing biomedical applications. © 2025 Elsevier B.V., All rights reserved.