This present study was aimed to investigate identification of potent actinomycetes that have the ability to produce bioactive compounds was isolated from the vermicast soil collected from Sirumali Hills South India. The ethyl acetate extraction of isolated Streptomyces showed high biological activity against human pathogens. The crude ethyl acetate extract was subjected to TLC to separate compounds. GC-MS and FTIR analaysis were carried out to reveal the structural elucidation of isolated compounds. The identification of bioactive compounds is based on the peak area and retention time. GC-MS chromatogram of extract showed the presence of six bioactive compounds, including saturated and unsaturated fatty acids. The antagonistic activity of Streptomyces sample might be due to the synergistic effect of the identified compounds. © 2020 Elsevier B.V., All rights reserved.