The study was proposed to find out the anti-tuberculosis activity of different solvent extracts of Solanum nigrum against Mycobacterium tuberculosis H37Rv strain. Solvent extraction of the plant material was done using various Solvents such as Acetone, Ethanol, Petroleum Ether, Chloroform, and Distilled Water. After the preparation of the inoculum, the extract was analyzed qualitatively for the presence of various phytochemicals such as Saponins, Tannins, terpenoids etc. The compound characterization was done using Gas column chromatography and the extracts biological activities have been studied. Mycobacterium tuberculosis activity was tested using Alamar Blue assay. All the extracts of Solanum nigrum had a significant effect against Mycobacterium tuberculosis at higher concentration but the acetone and ethanolic extract showed activity even at the lower concentration of 1.6µg/ml. GC-MS analysis showed the chemical compounds present in the extracts which were utilized for molecular docking using the Molegro virtual docking software against the standard drug targets 2CIG, 5BTC, and 1F8I. Ethanolic extract of Solanum nigrum showed significant anti-bacterial, anti-oxidant and anti-cancerous activity. Molecular docking studies with the proteins 2CIG, 5BTC and 1F8I proved that the chemical compounds obtained through GC-MS analysis have potent anti-tubercular activity by blocking these proteins. Ethanol and Acetone extracts showed moderate activity against E. coli strain and also for the DPPH assay which tends to show the antioxidant activity. © 2020 Elsevier B.V., All rights reserved.