The aim of this research was to study the influence of the composition of saturated fatty acids of biodiesel on the performance, emissions and characteristics of combustion in an engine with compression ignition. An increase in saturated fatty acids may eliminate biodiesel deficiencies. Various 55% mixtures of saturated fatty acids with diesel fuel were studied and compared. Five different combinations of blend were prepared using the following esters such as Palm oil methyl ester (POME), Coconut oil methyl ester (COME), Neem oil methyl ester (NOME), Mahua oil methyl ester (MOME), Jatropha oil methyl ester (JOME) and Pongamia oil methyl ester (PONOME). All five combinations (BD1-BD5) were tested and compared for physicochemical characteristics such as density, viscosity, iodine number, saponification number, energy consumption, thermal efficiency, exhaust gas temperature, toxic gas emissions. Five blended combinations showed viscosity around 5.13 to 5.56 centistokes. The Cetane number of the five blends were in the range of 52-56. The heating value of the five combinations was in the range of 38.92 to 40.65 MJ/kg. Iodine values were found to be in the range of 50-73. The brake thermal efficiency percentage were in the range of 27.15-27.4. All five blends found to emits less amount of Greenhouse gases. The results of the research state that all five combinations of biodiesel are unique, and they show very good performance properties as fuel along with less discharges toxic emissions. © 2023 Elsevier B.V., All rights reserved.