Two selective methods were developed and validated for the determination of Doxofylline in tablet dosage form. One method is based on UV Spectroscopy where UV spectra of Doxofylline were recorded. The absorption maximum (λ<inf>max</inf>) was observed at 274 nm which obeys Beer’s law over the concentration range from 2 to 20 µg/ml and shows linearity. The validation method proposed for Recovery studies was further confirmed at 50%, 100% and 150%. The percentage recovery values were 98% w/w, 100.5% w/w, 99.70% w/w, and 99.96% w/w, indicating good accuracy and reproducibility of the study. The other method is based on Reverse Phase HPLC using Methanol:Disodium phosphate in the ratio of 30:70 as a mobile phase and Phenomenox Luna C18 (5 micron, 250 × 4.6 mm) as stationary phase. The flow rate of Doxofylline was 1.0 ml/min, and the UV detector was operated at 274 nm. The method was validated for accuracy, linearity, precision, specificity, and limit of quantification. The recovery studies showed observed percentage recovery of Doxofylline ranging from 98.4 to 98.9%. The retention time of Doxofylline was 12.77 min. The developed method was precise and accurate, as evidenced by analytical data and recovery studies. © 2020 Elsevier B.V., All rights reserved.