To evaluate catheter-related infections in patients undergoing haemodialysis and assess the impact of an educational intervention and infection control bundle on CLABSI rates. This hospital-based interventional study was conducted in a dialysis unit in rural Puducherry, over six months with 19 patients on central venous catheters and 20 staff. The baseline CLABSI rate of 26.3 declined to 10.5 post-intervention. Enterococcus spp. And Coagulase-negative Staphylococcus were predominant isolates. Staff and patient compliance improved significantly (p < 0.001). Educational interventions and infection control bundles adherence effectively reduced CLABSI rates, emphasizing continuous training, surveillance and real-time feedback to sustain improvement and enhance patient's outcomes. © 2025 Elsevier B.V., All rights reserved.