Introduction: The remineralization of the initial carious area can be accomplished by therapeutic or biological methods rather than the conventional surgical approaches. One of the key fundamentals of a biological approach is the practice and application of remineralizing agents to the tooth. Objective: To assess the remineralizing potential of natural product, whey extract, and to compare it with the commercially available MI varnish and Xylitol mouthwash. Methods: 60 maxillary premolar teeth indicated for orthodontic extraction purpose was subjected to demineralization and were randomly divided into 3 groups of 20 samples in each group: Group 1 – Whey Extract, Group 2 – MI Varnish and Group 3 – Xy-litol Mouthwash. The whole experimental process was standardized by taking great attention on sectioning and regarding the treatment of groups with respective remineralizing agents and. All the samples were analyzed by using CFLSM (Confocal Laser Scanning Microscope). The results obtained were evaluated statistically. Results: The present study displayed that remineralization potential was maximum in Group 2 followed by Group 1 and finally Group 3 during 1st week and 2nd week but the remineralization potential during 4th week was improved in Group 1 followed by Group 2 and 3. Conclusion: Whey extract can be used as a natural remineralizing agent as a substitute to fluoride and has long-lasting remin-eralization potential compared to MI varnish and Xylitol mouth wash. © 2021 Elsevier B.V., All rights reserved.