Introduction: Platelet Rich Fibrin (PRF) is a favourable, absolutely autologous leukocyte and platelet concentrate which is being effectively used in numerous fields of dentistry and medicine. Objectives: Evaluation of the use of PRF gel for bone regeneration after surgical removal of bilateral mandibular 3rd molars – a comparative prospective study. Methods: This study consisting of a total of nine patients requiring bilateral trans alveolar third molar extractions. PRF was used from patient blood and placed in the test group and control group without PRF. After Placing the PRP Gel in the Socket region an Immediate orthopantomogram (OPG) was taken. All patient were recalled at regular intervals of 2nd week, 6th week, 10th week, 16th week and 20th-week post-op to take OPG’s. Comparing the bone regeneration results of the participants on the study side and control side with five months follow up were tabulated. Results: The parameter accessed was the bone density of the extraction socket on both the sides for each patient using 3D digital imaging software. There was a significant increase in bone density in test quadrant with PRF compared to the control side. Conclusion: It was concluded that PRF is helpful in the regeneration of bone after third molar surgery. © 2021 Elsevier B.V., All rights reserved.