Introduction: The TP53 gene is located on chromosome 17p13.1.P53 gene considered as the guardian of the genome belongs to a threemembered gene family p53, p63, and p73.1 p53 plays a vital role in human cancer and whose mutation was observed in nearly 50% of cancer all around the world. In the present study, genotype distribution was assessed in 147 infertile males, fertile males, and the associations of TP53 Arg72Pro polymorphism with hormonal and seminal parameters were investigated. Materials and methods: A total of 147 infertility men and 150 fertile men were taken for the study. Polymerase chain reaction (PCR) was carried out to amplify the exon 4 of the human p53 CODON 72 genotyping was performed by restriction fragments length polymorphism (RFLP). Results: In summary, the AA genotype and the A allele are significantly associated with azoospermic men when compared to fertile men. Conclusion: The study found preliminary evidence demonstrated that the TP53 gene Arg72 Pro polymorphism contributes significant association to male infertility. Aim and objective: To analyze the association of p53 gene polymorphism in male infertility cases in the study population. © 2021 Elsevier B.V., All rights reserved.