Introduction: Successful endodontic therapy depends on root canal disinfection and three-dimensional obturation of the root canal space. Complete removal of intracanal microorganisms and generates a promising environment for healing, prevents reinfection. Numerous root canal irrigants have been tried. Objective: This study was done to assess the antibacterial efficiency of neem, tulsi extract, Aleo vera, turmeric and 5% sodium hypochlorite against Enterococcus faecalis. Methods: Herbal extracts of neem, tulsi extract, Aleo vera, Curcuma longa. Agar plates were prepared using Tryptone soya broth to grow E. faecalis. The tested solutions (Group A: 5% NaOCl, Group B: neem, Group C: tulsi extract, Group D: Aleo vera, Group E: Curcuma longa) were added to the wells made on agar media. Agar diffusion test was accomplished. Plates were incubated for 24 hours at 37°C. Bacterial zones of inhibition were noted. Results: The data were statistically evaluated by Analysis of Variance (ANOVA) and post hoc comparison by Tuk-ey’s t-test. The maximum zone of inhibition against E. faecalis was seen with 5% NaOCl, followed by neem, tulsi extract, and Aleo vera showed similar antibacterial activity. The least zone of inhibition was seen with turmeric. Conclusion: 5% NaOCl presented the maximum antibacterial activity, and herbal products demonstrated significant antibacterial activity against E. faecalis and can be suggested as an alternative to NaOCl. © 2021 Elsevier B.V., All rights reserved.