Now a days, oxidative stress is one of the major disease around the world. The excess production of oxidants in the body are key source for development of different chronic diseases and are leading to damage the multiple organs' function, finally to cellular mortality. The natural remedy is the most important medicine to control the overproduction of oxidants and to obtain more antioxidants. Natural product have been using to treat diseases and as food supplements as nutrients. In this point of view, the current study was carried to evaluate the antioxidant activity of Spilanthes acmella root parts. The free radical scavenging activity was studied against 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide and hydroxyl free radicals using standard methods. The Spilanthes acmella root part extracts showed concentration dependent antioxidant activity on tested free radicals. The extracts showed modest effect on free radicals compared to the standard drug ascorbic acid and hydroalcoholic extract possesses more activity than other two extracts. The end results of current study clearly state that Spilanthes acmella root possess free radical scavenging activity and also confirms its' traditional value in traditional medicine. The further research is worthful on isolation of different bioactive molecules and different biological activities evaluation on Spilanthes acmella. © 2021 Elsevier B.V., All rights reserved.