This study investigates bio-oil production from blended citrus fruit peel using hydrothermal liquefaction. Fruit peel waste is abundant and can be converted into valuable bio-oil. Optimization of temperature, time, and biomass concentration revealed that time and temperature significantly affect yield. Maximum bio-oil yield was 29.4% at 280°C, 60 min, and 80g/200mL. GC-MS analysis detected hydrocarbons and alkanethiol compounds suitable for transportation fuel, though nitrogen and oxygen compounds reduced fuel standards. Produced bio-oil can be blended with conventional fuel after upgrading.