The natural waterbody such as rivers and lakes are able to do the purification process themselves, which has the primary dependant of absorption capacity and dissolution capacity of atmospheric oxygen from the surface of the water body. The various pollutants such as biological pollutants and chemical pollutants are broken-down into insignificant strength over a period of time by the growth of certain bacteria. This bacterial growth in the water is possible only with the absorbed and dissolved oxygen content in the water. This absorbed and dissolved oxygen content in the water bodies are based on the velocity of the stream, depth, discharge rate and temperature of the water, thus the self-depuration capacity of the water bodies are depended on the natural profiles and environmental factors of the waterbody and its location. This explains that the turbulent water will get purified by itself in much higher rate than the stagnant water, which tends to become septic because of the oxygen scarcity. The degree of self-depuration of a natural water body is based on the physiochemical and biological activities occurring in the system. Various parameters like basic physical properties, chemical properties such as pH, hardness, dissolved solids, mineral content, BOD, dissolved oxygen for the samples were taken along the Pamba River. The samples are taken from three zones of the Rivers viz. 1) Forest zone, 2) Residential zone and 3) Industrial zone. The water quality factors from the samples are validated against the desirable values as per IS 10500: 2012 and justifies the current status of the River and recommendations for its wellbeing. © 2021 Elsevier B.V., All rights reserved.