An efficient and easy synthesis of highly functionalized thiazole and pyridinone-substituted benzimidazole derivatives has been developed. The imino carbon of the benzimidazole derivative is coupled with the nitrogen atom of 1,4-dihydropyridinone and the aminothiazole-substituted acetic acid is condensed with two differently functionalized aniline, in two different reactions to form the amide linkages. These two amides and pyridinone-coupled benzimidazole are characterized by IR, 1H & 13C NMR and mass spectral studies. They are further evaluated for their antibacterial activity against the gram-positive bacterium Staphylococcus epidermidis and the fungus Saccharomyces cerevisiae by molecular docking method. All the three synthesized compounds had relatively lesser binding energy than what the standard drugs chloramphenicol and fluconazole have and may be considered as better inhibitors. © 2021 Elsevier B.V., All rights reserved.