Bionanotechnology approaches are used by researchers to create nanoparticles and nanomaterials in an environmental friendly and cost-effective manner. Because of biologically active plant secondary metabolites that aid in green synthesis, as well as their specific biological applications, biosynthesized nanoparticles are gaining traction. This work describes a simple, eco-friendly, dependable and cost-effective method for making silver nanoparticles from aqueous leaf extract of Psychotria octosulcata, as well as their anti-inflammatory properties in vitro. UV-Visible spectroscopy, Fourier Transform Infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning electron microscopy (SEM) and Zeta potential (ZP) were used to analyze the biosynthesized Psychotria octosulcata silver nanoparticles. Under a scanning electron microscope, the silver nanoparticles had a spherical shape and sizes ranging from 1 to 100 nm. The peaks from the Fourier Transform for the functional groups C=O, -C=C, C-H and OH in the infrared spectroscopy spectra of silver nanoparticles demonstrate the stability of the produced silver nanoparticles. The particles were crystalline and had a face-centered cubic structure with broad peaks in nature, according to the XRD studies. Protein denaturation was used to test the anti-inflammatory activity of the synthesized silver nanoparticles in vitro. © 2022 Elsevier B.V., All rights reserved.