Petroleum fuels emit hazardous pollutants into the atmosphere when used in Internal Combustion engines, endangering people, plants, and agriculture. There is currently a lot of study being done to develop a fuel substitute for petroleum and to raise the caliber of that fuel. DiEthyl Ethers (DEE) is a commonly used substance that can be used to enhance fuel characteristics and decrease nitrogen oxide poison gas. Engine testing was carried out on predictable Direct Injection (DI) engines with a diesel and biodiesel blend under various operating circumstances. In this work, the performance and emissions characteristics of a diesel engine running on a Pumpkin Seed Oil Methyl Ester Mixture (PSOME20) are being examined. Different DEE concentrations (10%, 15%, and 20%) were incorporated into the PSOME20 fuel. According to the results, the Brake Thermal Efficiency (BTE) of the PSOME20 fuel is 2.3 percent higher than that of the diesel, but the Brake Specific Fuel Consumption (BSFC) is reduced by 6.4 percent. When diethyl ether was combined with PSOME20, engine emissions of Hydrocarbon (HC), Carbon monoxide (CO), and smoke increased dramatically, while Nitrogen Oxide (NOx) emission was minimal in comparison to the other emission characteristics. © 2023 Elsevier B.V., All rights reserved.