Nanoparticles and plant phytochemicals are receiving great interest between materials and drug discovery studies. Hence, this work report the synthesis of zinc oxide nanoparticles (ZnO NPs) using Pashanabheda (Aerva lanata), which is employed as an antiurolithiatic agent. The selection of the Aerva lanata flower, a member of the Amaranthaceae family, as the source material was based on its ability to exhibit a wide range of genetic modifications in response to various environmental and geographical factors. The synthesized ZnO NPs were characterized by UV, FTIR, XRD and scanning electron microscopy. Due to flower's chemical make-up and presence of several functional groups in ZnO nanoparticles were found to be effective at bio-reduction and capping. Antibacterial assay against B. subtilis, S. aureus, K. pneumonia and Pseudomonous using ZnONPs of A. lanata flower exhibited excellent inhibition zones between 11 mm and 18 mm for 100 μg/mL. © 2023 Elsevier B.V., All rights reserved.