Nanotechnology has gained prominence in various fields such as medicine, electronics, waste treatment and defence sectors. Aegle marmelos, commonly known as Bael, is used in ancient Siddha and Ayurveda medicines from time immemorial and is considered as one of the most sacred plants in India. An attempt has been made in this research work to synthesize silver nanoparticles with the aid of a novel, non-toxic, eco-friendly and biological method using Aegle marmelos leaf extract for the application as a topical ointment to prevent infection against burns and wounds. Hydrogel loaded with metal nanoparticle have great importance for many applications in the biomedical and biotechnological field. The present study is aimed at the biosynthesis of silver nanoparticle-based hydrogel of Aegle marmelos (Mahavilvam) extract for topical delivery in wound dressing. Further, the characterization study was carried out on biosynthesised silver nanoparticle-loaded hydrogel by using UV-Visible Spectrophotometer, Fourier Transform Infrared Spectroscopy and SEM analysis. The antimicrobial resistance against Escherichia coli and Staphylococcus aureus was measured. © 2024 Elsevier B.V., All rights reserved.