Malassezia spp is a normal inhabitant of human skin. In certain predisposing conditions, they proliferate and grow in hyphal forms without inflicting inflammation as in pityriasis versicolor and may involve in the pathogenesis of disease with noticeable inflammation such as psoriasis, seborrheic dermatitis and atopic dermatitis. Malassezia spp accounted approximately 60-90% of the total cutaneous fungal microflora. A clear understanding about the distribution of Malassezia spp in healthy individuals is necessary to gain the knowledge in the involvement of Malassezia as pathogen from commensals. A total of 120 healthy men and 120 healthy women without any skin disease or who were not treated for Malassezia associated skin disease were selected for study. The specimen of skin scrapings was collected from forehead, chest, neck and back from each subject totaling 880 sites. Phenotypically the samples were processed. The isolation rate of Malassezia from 240 healthy individuals was 41% (N=99). The positive culture rate in healthy individuals was high in the age group of 21-30 (32%). In healthy individuals, M. globosa was the most common species with 154 isolates (53%) followed by M. furfur 82 (28%), M. pachydermatis 33(11.3%), M. sympodialis 16(5.4%), M. slooffiae 4(1.3%), M. restricta 2(0.6%) and M obtusa 1(0.3%). The overall colonization rate of Malassezia spp in healthy individuals found in this study was 41%. M. globose was identified as the most common species (53%). © 2023 Elsevier B.V., All rights reserved.