Introduction: During medicolegal situation, mass disasters or any conditions requiring forensic identi ication, the skull bones can be exposed to radiation to obtain lateral cephalometric radiographs.. The landmarks present in the radiographs can be used to determine the sex of the individual after measurement. Aim: The aim of our study is to develop a formula to identify sex of the population of Salem district. Materials and methods: A cross-sectional study was conducted using 100 digital lateral cephalometric radiographs of 6 to 30 years old population of Salem district. All the lateral cephalometric radiographs will be traced and analyzed digitally by using a standardized Carestream CS8100 SC software. Result: The mean values of males higher for variables such as AO (A to Occlusal Plane), BO (B to Occlusal Plane), Chin thickness (Pog' to N-B) than females. This study showed a overall accuracy of 92.6% for determination of sex using lateral cephalometric radiographs. Conclusion: This study was able to develop formula for gender identi ication in Salem population. © 2024 Elsevier B.V., All rights reserved.