Severe environmental implications, such as those connected to greenhouse gas emissions and widespread pollution, have resulted from the unsustainable demand for fossil fuels. In order to find and implement technological, sustainable, and cost-effective alternatives to fossil fuel production and use, it is necessary to first identify the primary challenges originating from these activities. The significant potential for their production from various types of biomass has led to a rise in their use in recent years, and this has coincided with the advent of current technological breakthroughs in the field. Scenedesmus dimorphus The energy sector has a strong interest in the process of making biodiesel from and spirulina oil because of its potential to provide clean and sustainable energy.The combustion, emission, vibration and noise characteristics of Scenedesmus Dimorphus and spirulina based microalgae biodiesel were studied in this study, both with and without the addition of nanoparticles and hydrogen. Hydrogen and nanoparticles were employed at a rate of 5 L/min and 100 ppm respectively. Biodiesel blends B10 (5 % spirulina + 5 % Scenedesmus Dimorphus + 90 % diesel) and B20 (10 % spirulina + 10 % Scenedesmus Dimorphus + 90 % diesel) were used in this study. The test fuels prepared from biodiesel, hydrogen and nanoparticles has been compared with conventional diesel.The experiment was carried out on four stroke diesel engine at varying load conditions ranging from 0 % to 100 % at an interval of 25 %. Incorporation of nanoparticles and hydrogen on microalgae based biodiesel reduces the emission, vibration and noise generated by the engine. © 2023 Elsevier B.V., All rights reserved.