Dioscorea oppositifolia, commonly known as Chinese yam, is a member of the Dioscoreaceae family which comprises of over 600 species. Dioscorea plants are known to contain various bioactive compounds including steroids, clerodane diterpenes, quinones, cyanidins, phenolics, diarylheptanoids and nitrogen compounds. The tubers and roots of Dioscorea species are particularly rich in steroidal sapogenins, primarily diosgenin along with volatile compounds. This study aimed to assess the in vitro antidiabetic and antioxidant properties of Dioscorea oppositifolia extract. The antidiabetic potential was evaluated using α-amylase and α-glucosidase inhibition assays while antioxidant activity was assessed through DPPH radical scavenging and ferric-reducing antioxidant power (FRAP) assays. The extract demonstrated significant inhibition of α-amylase and α-glucosidase enzymes, indicating its potential to modulate postprandial blood glucose levels. Additionally, the extract exhibited strong antioxidant activity by effectively scavenging DPPH radicals and increasing the FRAP value. These findings highlight the potential of Dioscorea oppositifolia as a natural source of antidiabetic and antioxidant agents, supporting further investigation into its therapeutic applications for diabetes and oxidative stress-related disorders. © 2025 Elsevier B.V., All rights reserved.