Introduction: Infertility poses significant challenges to couples, impacting emotional well-being. Vitamin D's role in reproductive health, particularly its potential influence on Anti-Mullerian Hormone (AMH) production, remains a subject of debate. Previous studies present conflicting findings regarding the relationship between vitamin D and AMH levels. Objectives: This study aimed to elucidate the correlation between serum vitamin D, AMH, and Follicle-Stimulating Hormone (FSH) levels in women facing infertility. The research sought to assess variations in vitamin D, AMH, and FSH concentrations, exploring their potential roles in infertility. Materials and Methods: The study involved 120 infertile women aged 22-43 years. Serum samples were collected and analyzed for vitamin D, AMH, and FSH levels. Statistical analyses, including one-way ANOVA and chi-square tests, were employed to examine associations. Results: Among participants, 60% exhibited vitamin D deficiency, and no significant correlation was found between vitamin D levels and AMH across different age groups. The study observed variations in AMH and FSH levels, emphasizing the complex interplay in infertility. Conclusion: This investigation underscores the prevalence of vitamin D deficiency in infertile women and challenges the purported direct link between vitamin D and AMH. Results indicate a nuanced relationship, necessitating further research to unravel the intricate dynamics of vitamin D's impact on reproductive markers in infertility. © 2024 Elsevier B.V., All rights reserved.